Glutathione Conjugation
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چکیده
منابع مشابه
Binding and glutathione conjugation of porphyrinogens by plant glutathione transferases.
Overexpression in Escherichia coli of a tau (U) class glutathione transferase (GST) from maize (Zea mays L.), termed ZmGSTU1, caused a reduction in heme levels and an accumulation of porphyrin precursors. This disruption was highly specific, with the expression of the closely related ZmGSTU2 or other maize GSTs having little effect. Expression in E. coli of a series of chimeric ZmGSTU1/ZmGSTU2 ...
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We have examined the catalytic activity of glutathione .S'-transferases (GST) in the conjugation of busulfan with glutathione (GSH) in human liver cytosol. purified human liver GST. and cDNA-expressed GST-al-1. Human liver microsomes and cytosol were incubated with 40 /IM busulfan and 1 m\i (.SII. Cytosol catalyzed the formation of the GSH-husulfan tetrahydrothiophenium ion i III I ' i in a con...
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The reversibility of the conjugation reaction of the diuretic drug ethacrynic acid (EA), an a,~-unsaturated ketone, with glutathione and glutathione S-transferase PI-1 (GST PI-I) has been studied. When the glutathione conjugate of EA was incubated with a 5-fold molar excess of Nacetyi-L-cysteine or GST PI-I, a time-dependent transfer of EA to N-acetyI-L-cysteine or GST PI-1 was observed. With i...
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Glutathione epoxide transferase has been isolated in homogeneous form from the livers of male Sprague-Dawley rats. The enzyme is active in the conjugation of glutathione with a large number of compounds bearing an ethylene oxide ring in the terminal portion of an alkyl chain. The enzyme has a molecular weight of 40,000 and is composed of 2 subunits. Several proteins which catalyze the same reac...
متن کاملGlutathione conjugation of chlorambucil: measurement and modulation by plant polyphenols.
Chlorambucil (CMB), an anticancer drug, was cytotoxic at concentrations of 5-20 microM to human colon adenocarcinoma cells. It inhibited [14C]thymidine uptake in a dose-dependent manner. Both effects were potentiated by simultaneous exposure of the cells to 10 microM plant polyphenols. In an attempt to explain the possible mechanism of action of the polyphenols in relation to these observations...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1971
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.47.1.10